Planta Medica, Table of Contents Planta Med 1979; 35(4): 308-315DOI: 10.1055/s-0028-1097222 Research Articles © Georg Thieme Verlag Stuttgart · New YorkTLX–5 Lymphoma Cells in Rapid Screening for Cytotoxicity in Brucea ExtractsJ. David Phillipson, Fikria A. Darwish Department of Pharmacognosy, The School of Pharmacy, University of London. United Kingdom. Recommend Article Abstract PDF Download Buy Article Abstract A rapid test for cytotoxicity utilising the in vitro uptake of 3H–thymidine into TLX–5 mice lymphoma cells has been used as an aid to the detection and isolation of active compounds from the roots of Brucea javanica (Simaroubaceae). An ethanol extract was partitioned between chloroform and water and further fractionation of the active chloroform extract obtained by column and thin–layer chromatography. At each stage the resulting fractions were tested for cytotoxicity against TLX–5 mice lymphoma cells. Bruceine A was isolated from a fraction having cytotoxic activity. The IDSO values of bruceine A and of podophyllotoxin (used as a control) against TLX–5 cells were determined as 0.031 μg/ml and 6 μg/ml, respectively. Key Word Index Brucea javanica - Simaroubaceae - Cytotoxicity Screening - TLX–5 Mice Lymphoma - Bruceolides PDF (733 kb)
